Тред №634153

Вот мое CV, которое я только что выложил на linkedin.com и сегодня начну выкладывать на разные сайты, типа biospace.com, indeed.com, и буду посылать рекрутерам и компаниям, которым, я думаю, нужна эффективная технология клонирования. Про русику, как вы видите, я здесь не пишу, как и про очень многие другие вещи. Пардон за English, на русский перевод пока времени нет. Но я сделаю его обязательно, потому что, даже когда я начну работать в биотех. компании в США, я буду параллельно искать работу в России. Я хотел бы, чтобы мои технологии работали на Россию.


Yuri M. Romantchikov, Ph.D.

OBJECTIVE

To use my patented cloning technology at a company that needs to clone “unclonable” DNA and to create fully representative DNA libraries including toxic, unstable, repetitive sequences. To use my proprietary high-density microarray and massive-parallel DNA synthesis technologies at such a company.

EXPERIENCE SUMMARY

• Chief Scientific Officer and one of the founders of Gene Source Inc., a genomics company specializing in DNA cloning and synthesis.  
• Prior to that, academic researcher studying molecular mechanisms of aging and cancer.
• Inventor of a variety of technologies ranging from DNA cloning and high-density microarrays to water desalination.
• Author of 4 theories of ontogenesis, aging and cancer and a theory of origin of life on Earth.
• Extensive knowledge of biology, physics and engineering.
• M.Sc. in Engineering Electrophysics, Ph.D. in Genetics.

ACCOMPLISHMENTS

• Invented DNA cloning technology with cloning efficiency more than 1,000 times higher than that of other methods, with no size bias for short inserts, with no background of colonies without inserts and with ability to clone toxic genes and direct or inverted repeats.
• Invented massive-parallel DNA synthesis technology utilizing patented cloning technology that provides more than 1000-fold cost reduction of DNA synthesis and allows quick and efficient synthesis of sequence-confirmed DNA molecules up to several million basepairs.
• Invented 2 subcloning methods that allow quick and efficient gene transfer from one plasmid to another without introduction of long recombination sites into the plasmids.
• Invented method of full-length cDNA synthesis utilizing thermostable reverse transcriptase with proofreading activity.
• Invented method of preferential cloning of long DNA sequences (such as full-length cDNAs).
• Invented high-density microarray technology that allows high-throughput, low cost DNA sequencing and screening of drug candidates with no side effects.
• Invented concentrating blocks for biochemical or chemical compounds without change of solvent and any physical or chemical treatment.
• Invented very low cost water desalination technology that can be used anywhere on a seashore.
• Cloned main gene target of mouse E2F transcription factor and 5 high affinity gene targets of tumor suppressor p53 from human and mouse genomes.
• Invented method of artificial telomere elongation without telomerase in normal human cells.
• Author of 4 alternative theories of ontogenesis, aging and cancer.
• Author of theory of origin of life on Earth in hot and hydrophobic conditions, our hydrophilic life form being only an offshoot of ancient hydrophobic life that may still exist in the Earth crust.

SKILLS & STRENGTHS

•  Creativity and inventiveness  •  Complex problem solving  •  Focused on result  •  Integrity  •  Hands on  •  Self-motivated  •  Work well under pressure  •  Team Leader  •  Mentorship  •  Patenting proficiency  •  Project management  •  Process development  •  Speak Russian  •  Cloning ‘unclonable’ DNA  •  Quick and efficient subcloning  •  Genomic and cDNA library construction and screening  •  Sequencing  •  Full-length cDNA synthesis  •  PCR, RT-PCR and qRT-PCR  •  Site directed mutagenesis  •  EMSA  •  Differential display  •  Northern, Southern and Western blotting  •  In vitro transcription and translation  •  DNA/RNA/protein purification  •  DNA/RNA/protein electrophoresis  •  HPLC  •  Gel filtration  •  ELISA  •  Immunoprecipitation  •  Immunofluorescence  •  Bacterial and mammalian cell culture  •  Microarrays  •  Drug screening  •  Oncogenes and tumor suppressors  •  Telomeres and telomerase  •  DNA/RNA/Protein software (BLAST Geneious BioToolKit Cn3D etc.)  •  Mathcad  •  Fortran  •  HTML

PROFESSIONAL EXPERIENCE

Gene Source Inc., Germantown, MD......................May 1997 – Present
Chief Scientific Officer

Invented technologies that formed scientific basis of Gene Source Inc.  Led development, patenting, licensing and commercialization of these technologies.

Memorial Sloan-Kettering Cancer Center, New York, NY..........Aug 1994 – Apr 1997
Research Fellow

Invented method of cloning gene targets of transcription factors and DNA binding proteins.  Using this method:

• Cloned main gene target of mouse E2F transcription factor that regulates cell cycle.
• Proved that cell-cycle-dependent transcription of mouse Histone H3 gene is regulated by several E2F sites at its 3’ end.
• Cloned high affinity gene targets of tumor suppressor p53 from human (2 genes) and mouse (3 genes) genomes.

UT Southwestern Medical Center, Dallas, TX.............................Jun 1992 – Aug 1994
Research Fellow    

• Invented method of artificial telomere elongation (without telomerase) in normal human cells – by expressing reverse transcriptase and RNA with CCCUAA repeats.
• Determined DNA binding sites associated with wild type and mutant p53, cyclins and cyclin-dependent kinases, using PCR-based selection method (CASTing).
• Analyzed human telomerase specificity, which provided a breakthrough in understanding mechanisms of telomerase work.

Institute of Gene Biology, Moscow, Russia..................................Oct 1991 – Jun 1992
Research Fellow

• Participated in cloning and sequencing of 2 developmentally expressed Xenopus genes.

All-Union Cancer Research Center, Moscow, USSR....................Jan 1989 – Oct 1991
Research Fellow

• Proposed 4 alternative theories of ontogenesis, aging and cancer, all utilizing the fact that genes responsible for aging and immortalization of cells are involved in regulation of gene activity.
• Led team testing these theories.

EDUCATION

Ph.D. in Genetics, Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Thesis:  "Theoretical Analysis of Aging and Immortalization of Animal Cells: Possible Role of Nuclear Oncogenes".
M.Sc. in Engineering Electrophysics, Novosibirsk Electro-Technical Institute, Novosibirsk, USSR.

PATENTS

Romantchikov Yuri: Methods for insertion of nucleic acids into circular vectors. US Patent 6825011.

Romantchikov Yuri: Improved methods for insertion of nucleic acids into circular vectors. EPO Patent 1141239-A1.

Romantchikov Yuri: Improved methods for insertion of nucleic acids into circular vectors. PCT Patent WO/2000/036088.

PUBLICATIONS

1. Романчиков Ю.М. 1992. Ключевая Роль Ядерных Прото-Онкогенов в Старении и Иммортализации Клеток Животных. Известия Российской Академии Наук, Сер. Биол., No 4, 985-988.

2. Романчиков Ю.М. 1991. Возможные Механизмы Действия Факторов Роста и Старение Клеток. Генетика 27, No 11, 1369-1377.

3. Романчиков Ю.М. 1991. Триггерный Подход к Пролиферации, Старению и Иммортализации Клеток Животных. Генетика 27, No 10, 1223-1230.

4. Романчиков Ю.М. 1991. Гипотеза об Экстрахромосомных Регуляторных Генах. Успехи Соврем. Биологии 111, No 5, 643-653.

5. Романчиков Ю.М. 1991. Онтогенез животных с позиции отсутствия самоподдержания синтеза регуляторных белков. Генетика 27, No 10, 1214-1222.

6. Романчиков Ю.М. 1991. Факторы Роста, Вторичные Мессенджеры и Онкогены. Успехи Соврем. Биологии 111, No 1, 19-33.

7. Романчиков Ю.М. 1988. Иерархия Регулонов: Связь с Делением и Дифференцировкой Клеток. Успехи Соврем. Биологии 105, No 3, 406-422.